In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides.
نویسندگان
چکیده
Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (approximatley 0.05-micrometer) approximatley 10 nm-diameter filaments. These results demonstrate that the triplet polypeptides are components of neural IF and that the 68,000-dalton polypeptide is an IF structural protein.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 79 3 شماره
صفحات -
تاریخ انتشار 1982